Application

Anti-XPD, clone 22TF2-2F6, Cat. No. MABE1819, is a highly specific mouse monoclonal antibody that targets human General transcription and DNA repair factor IIH helicase subunit XPD and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected XPD in Western Blotting applications (Alekseev, S., et. al. (2017). Mol Cell. 65(3):504-514.e4).

Immunocytochemistry Analysis: A representative lot detected XPD in Immunocytochmeistry applications (Vermeulen, W., et. al. (2000). Nat Genet. 26(3):307-13). .

Immunoprecipitation Analysis: A representative lot immunoprecipitated XPD in Immunoprecipitation applications (Coin, F., et. al. (1998). Nat Genet. 20(2):184-8; Seroz, T., et. al. (2000). Nucleic Acids Res. 28(22):4506-13).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

General transcription and DNA repair factor IIH helicase subunit XPD (UniProt: P18074; also known as EC: 3.6.4.12, TFIIH subunit XPD, Basic transcription factor 2 80 kDa subunit, BTF2 p80, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH 80 kDa subunit, TFIIH p80, Xeroderma pigmentosum group D-complementing protein) is encoded by the ERCC2 (also known as XPD, XPDC) gene (Gene ID: 2068) in human. XPD is an ATP-dependent 5′-3′ DNA helicase component of the general transcription and DNA repair factor IIH (TFIIH) core complex that is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA. Its ATP-binding region is localized to amino acids 7-283 and its nuclear localization is in amino acids 682-695. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH plays an essential role in transcription initiation. When the pre-initiation complex has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail of the largest subunit of RNA polymerase II by CDK-activating kinase (CAK) complex controls the initiation of transcription. XPD acts by forming a bridge between CAK and the core-TFIIH complex. Mutations in ERCC2 gene are known to cause Xeroderma pigmentosum complementation group D that is characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. The skin develops marked freckling and other pigmentation abnormalities.

Immunogen

Epitope: C-terminus

Ovalbumin-conjugated linear peptide corresponding to 32 amino acids from the C-terminal region of human General transcription and DNA repair factor IIH helicase subunit XPD (XPD/ERCC2).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected XPD in HeLa cells.

Specificity

Clone 22TF2-2F6 detects General transcription and DNA repair factor IIH helicase subunit XPD (ERCC2) in human cells. It targets an epitope with in 32 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

86.91 kDa calculated.